Download ASCP : ASCP-MLT Questions & Answers as PDF & Test Software
Updated: Sep 02, 2026
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ASCP ASCP-MLT Exam Overview:
| Certification Vendor: | ASCP Board of Certification (ASCP BOC) |
|---|---|
| Exam Name: | MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) |
| Exam Number: | ASCP-MLT |
| Exam Duration: | 150 minutes |
| Related Certifications: | PBT(ASCP) MLT(ASCPi) MLS(ASCP) |
| Exam Price: | $235 USD (standard); $215 USD (reduced for eligible regions) |
| Passing Score: | 400 (scale 0-999) |
| Available Languages: | English |
| Exam Format: | Multiple-choice questions, Computer Adaptive Testing (CAT) |
| Real Exam Qty: | 100 |
| Certificate Validity Period: | 3 years (renewable via CMP) |
| Recommended Training: | ASCP BOC MLT Content Guideline ASCP Recommended Reading List |
| Exam Registration: | ASCP BOC Official Application Portal Pearson VUE Testing Center Scheduling |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Proctored computer-based testing at authorized Pearson VUE test centers |
| Pre Condition: | Associate degree or 60 semester hours from accredited institution + completion of NAACLS/ABHES-accredited MLT program OR approved education + clinical experience; multiple eligibility routes available |
| Official Syllabus URL: | https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT |
ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Immunology and Serology | 5-10% | - Infectious disease serology - Immunologic testing methodologies - Autoimmune and immunodeficiency disorders - Immune system principles and components |
| Topic 2: Microbiology | 15-20% | - Bacteriology and antimicrobial susceptibility - Mycology, parasitology, and virology - Specimen collection and culture techniques - Identification of pathogenic microorganisms |
| Topic 3: Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes - Toxicology and liver/kidney function tests - Electrolytes, acid-base balance, and blood gases |
| Topic 4: Blood Banking (Immunohematology) | 15-20% | - Antibody detection and identification - Transfusion reactions and adverse events - Blood components and storage - Blood group systems and antigens - Compatibility testing |
| Topic 5: Urinalysis and Other Body Fluids | 5-10% | - Semen and amniotic fluid testing - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis - Urine formation and composition |
| Topic 6: Hematology | 20-25% | - White blood cell morphology and diseases - Red blood cell physiology and disorders - Routine and automated hematology procedures - Hemostasis and coagulation testing |
| Topic 7: Laboratory Operations | 5-10% | - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes - Laboratory safety and regulatory compliance - Quality control and quality assurance |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
Cholesterol levels do not fluctuate as triglycerides, fatty acids, lipoproteins, and chylomicrons do after an individual eats a meal. This is the reason that patients are told to fast; the laboratory needs a fasting sample to determine a lipid profile or panel which includes the latter four components.
Which of the following lipid tests is LEAST affected by the fasting status of the patient?
A. Triglycerides
B. Lipoproteins
C. Cholesterol
D. Chylomicrons
E. Fatty acids
Question 2
When making a platelet concentrate, the proper procedure is to start with a low centrifugation of the whole blood bag. After the plasma is removed, it is centrifuged again at a higher speed to separate the platelet portion from the plasma portion.
Blood bank
The following steps must be followed in preparation of a platelet concentrate:
A. Whole blood centrifuged at high speed - plasma separated then centrifuged at low speed
B. Whole blood centrifuged at low speed - plasma separated then centrifuged at low speed
C. Whole blood centrifuged at high speed - plasma separated then centrifuged at high speed
D. Whole blood centrifuged at low speed - plasma separated then centrifuged at high speed
Question 3
Waived tests are those considered to have an insignificant risk of erroneous results. Which of the following is NOT an example of a waived test?
A. fructosamine
B. spun hematocrit
C. blood glucose by home meters
D. potassium
Question 4
Normocytic- G6PD deficiency, Malaria
Microcytic- Iron deficiency, Thalassemia
Macrocytic- Chronic Liver Disease, Vitamin B12 deficiency
Hematology
Match the disease conditions with appropriate red cell size classification
1. G6PD deficiency, Malaria
2. Iron deficiency, Thalassemia
3. Chronic Liver Disease, Vitamin B12 deficiency
A. Normocytic
B. Microcytic
C. Macrocytic
Question 5
Failure to deliver glucose drawn in a serum separator tube (SST) to the laboratory within the recommended time will cause:
Question options:
A. both A and C
B. glycolysis
C. a falsely decreased glucose value
D. a falsely increased glucose value
Solutions:
| Question 1 Answer: C | Question 2 Answer: D | Question 3 Answer: D | Question 4 Answer: A,B,C | Question 5 Answer: A |
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